[Changes in number of emergency medical service deployments in an urban area during the first COVID-19 pandemic-related contact restriction phase]. / Veränderungen der rettungsdienstlichen Einsatzzahlen in einer Metropolregion während der ersten COVID-19-Pandemie-bedingten Kontaktbeschränkungsphase.

BACKGROUND: To contain the coronavirus disease (COVID-19) pandemic, public life was reduced through contact restriction measures (referred to as "lockdown" in the further course for reading simplicity), among other things to make health system resources available for the treatment of COVID-19 patients. In parallel, a decrease in emergency patients was observed in the public health system. METHODS: For two 10-week periods before and during the lockdown, ambulance service deployment rates were analysed in 6 ambulance service areas for 6 tracer diagnoses. Random effects were minimised by comparing the results with the corresponding 2018 and 2019 time periods and a calculated expected value. RESULTS: For emergency ambulance service calls, there was a reduction in call numbers (-16%) during the lockdown. A 20% reduction for the categories cardiac and cerebral ischaemia was found. In the urban area, the reduction in cardiac ischaemia was less pronounced at 14% than in the surrounding area at 23%. The deployment figures for intoxications decreased by 27% and for psychiatric emergencies by 16%. CONCLUSION: The public ambulance service was not overwhelmed by the COVID-19 pandemic; there was a decrease in depolyments during the lockdown. For the reduction in cerebral or cardiac ischaemias, the explanatory models for the influence of the lockdown are missing. Further studies on the utilisation behaviour of the ambulance service during a lockdown appear necessary in order to detect potentially fatal reductions in utilisation for the patient outcome and to be able to counteract them through education.

Reply to Fabbris et al. A Viable Alternative. Comment on "Kohmer et al. Self-Collected Samples to Detect SARS-CoV-2: Direct Comparison of Saliva, Tongue Swab, Nasal Swab, Chewed Cotton Pads and Gargle Lavage. J. Clin. Med. 2021, 10, 5751".

We thank Fabbris et al. for their remarks [...].

[Occupationally acquired SARS-CoV-2 infections among healthcare personnel in Frankfurt am Main from March to August 2020]. / Beruflich erworbene SARS-CoV-2-Infektionen bei medizinischem Personal in Frankfurt am Main von März bis August 2020.

BACKGROUND: Standardised surveillance of COVID-19 infections among healthcare personnel during the current pandemic was and is not available. In particular, the proportion of occupational infections among healthcare personnel and which workers among them are most at risk remains unclear. OBJECTIVES: The aim of this study was to analyse the reported COVID-19 cases among healthcare personnel in Frankfurt/Main during the first six months of the pandemic, to determine the number of occupational infections and thus to allow a better interpretation of the data published by the Robert Koch Institute. METHODS: Data from the Frankfurt/Main Health Protection Authority was analysed for the period from 1 March to 31 August 2020, and healthcare personnel were recruited for a cross-sectional survey. Three subgroups were defined and analysed according to whether the infectious contact occurred at work, in private or in an unknown setting. RESULTS: Healthcare personnel accounted for 11.8% (319/2700) of all reported COVID-19 cases in Frankfurt/Main during the period studied. In the survey, 47.2% of respondents reported that their infection was acquired in the workplace. There was an association of contact with COVID-19 patients as well as employment in the internal medicine ward and a reported work-related infection. Also apparent was an association between suspected occupational infections and consequently filed reports for alleged occupational disease. DISCUSSION AND CONCLUSION: Health protection authorities are in a position to collect relevant data on work-related transmissions in healthcare occupations and workplaces and should generate standardised data on infected healthcare personnel. This data is necessary to take targeted infection control and prevention measures that protect healthcare personnel and their patients.

[The SARS-CoV-2 Pandemic in Long-Term Care Facilities for the Elderly: Analysis of Data from Frankfurt am Main, Germany, March 2020 - September 2021]. / Die SARS-CoV-2-Pandemie in Altenpflegeheimen: Erkenntnisse und Analysen in Frankfurt am Main von März 2020 bis September 2021.

BACKGROUND: Residents in long-term care facilities (LTCF) are particularly vulnerable during the SARS-CoV-2 pandemic. In the first wave of the pandemic in many countries, 30-70% of all deaths from or with SARS-CoV-2 were LTCF residents, although their proportion in the population is typically less than 1%. Findings from LTCFs in Frankfurt am Main (March 2020-September 2021) are presented below and discussed in terms of necessary improvements. MATERIAL AND METHODS: The reports of positive PCR tests for SARS-CoV-2 in residents and staff of the LTCF in Frankfurt am Main and their symptoms were descriptively evaluated. In addition, the total deaths in nursing homes from 2018 to June 2021 were surveyed per quarter. RESULTS: In the first pandemic wave (March-May 2020), 111 SARS-CoV-2-positive LTCF residents were reported to the Public Health Department in Frankfurt am Main, of whom 40% were asymptomatic, 48% were hospitalized, and 23% died. In the subsequent pandemic phases through September 30, 2021, additional 1196 residents infected with SARS-CoV-2 were reported, with most of them being asymptomatic (70%); they were hospitalized less frequently (27%). Mortality was also lower (17.6%). Overall mortality in LTCF was 7.6% higher in 2020 than in 2019 and 1.1% higher than in the "flu year" of 2018. DISCUSSION: In contrast to the first wave, when only a few LTCF residents contracted COVID-19, in the second pandemic wave in autumn/winter 2020/21, with high incidences in the general population, SARS-CoV-2 outbreaks in LTCF in Frankfurt could not be prevented, despite extensive hygiene, infection prevention, and contact mitigation measures (including visitor restrictions) that massively limited residents' quality of life and their personal rights. Only when vaccination rates increased among residents and staff from April 2021 onwards, there were no massive outbreaks. To better protect LTCF residents, an appropriate balance was called for between protecting against infection and avoiding collateral damage by maintaining the freedom and quality of life of nursing home residents as best as possible.

SARS-CoV-2 screening strategies for returning international travellers: Evaluation of a rapid antigen test approach.

BACKGROUND: International travel poses the risk of importing SARS-CoV-2 infections and introducing new viral variants into the country of destination. Established measures include mandatory quarantine with the opportunity to abbreviate it with a negative rapid antigen test (RAT). METHODS: A total of 1,488 returnees were tested for SARS-CoV-2 with both PCR and RAT no earlier than 5 days after arrival. We assessed the sensitivity and specificity of the RAT. Positive samples were evaluated for infectivity in vitro in a cell culture outgrowth assay. We tracked if participants who tested negative were reported positive within 2 weeks of the initial test. RESULTS: Potential infectiousness was determined based on symptom onset analysis, resulting in a sensitivity of the antigen test of 89% in terms of infectivity. The specificity was 100%. All positive outgrowth assays were preceded by a positive RAT, indicating that all participants with proven in vitro infectivity were correctly identified. None of the negative participants tested positive during the follow-up. CONCLUSIONS: RAT no earlier than the 5th day after arrival was a reliable method for detecting infectious travellers and can be recommended as an appropriate method for managing SARS-CoV-2 travel restrictions. Compliance to the regulations and a high standard of test quality must be ensured.

Self-Collected Samples to Detect SARS-CoV-2: Direct Comparison of Saliva, Tongue Swab, Nasal Swab, Chewed Cotton Pads and Gargle Lavage.

Testing for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by RT-PCR is a vital public health tool in the pandemic. Self-collected samples are increasingly used as an alternative to nasopharyngeal swabs. Several studies suggested that they are sufficiently sensitive to be a useful alternative. However, there are limited data directly comparing several different types of self-collected materials to determine which material is preferable. A total of 102 predominantly symptomatic adults with a confirmed SARS-CoV-2 infection self-collected native saliva, a tongue swab, a mid-turbinate nasal swab, saliva obtained by chewing a cotton pad and gargle lavage, within 48 h of initial diagnosis. Sample collection was unsupervised. Both native saliva and gargling with tap water had high diagnostic sensitivity of 92.8% and 89.1%, respectively. Nasal swabs had a sensitivity of 85.1%, which was not significantly inferior to saliva (p = 0.092), but 16.6% of participants reported they had difficult in self-collection of this sample. A tongue swab and saliva obtained by chewing a cotton pad had a significantly lower sensitivity of 74.2% and 70.2%, respectively. Diagnostic sensitivity was not related to the presence of clinical symptoms or to age. When comparing self-collected specimens from different material, saliva, gargle lavage or mid-turbinate nasal swabs may be considered for most symptomatic patients. However, complementary experiments are required to verify that differences in performance observed among the five sampling modes were not attributed to collection impairment.

[COVID-19 with respect to occupational healthcare and public health services]. / COVID-19 im Hinblick auf Arbeitsmedizin und Öffentlichen Gesundheitsdienst.

BACKGROUND: Healthcare personnel (HCP) have an occupation-related risk of an infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and can transmit COVID-19 to patients. The related occupational disease is the BK 3101. OBJECTIVE: The frequency of suspected claims of occupation-related SARS-CoV­2 infections and confirmed claims of COVID-19 occupational diseases, reporting obligations under the German Infection Protection Act (IfSG) and mandatory notification requirements of physicians. MATERIAL AND METHODS: Selective literature search with special respect to data of accident insurance institutions regarding occupation-related SARS-CoV­2 infections among HCP. RESULTS: The SARS-CoV­2 pandemic also represents a challenge for occupational healthcare and the public health service. In recent months an increased number of suspected cases of an occupational disease (BK 3101) associated with COVID-19 were registered at the accident insurance institutions as well as notifications of accidents at work. The public health service handles registrations under the IfSG and coordinates the tracking of contact persons. CONCLUSION: Occupation-related SARS-CoV­2 infections are a real reason for concern. The registration data of the accident insurance institutions confirm high case numbers. Preventive measures, such as wearing personal protective equipment (PPE) and COVID-19 vaccinations significantly reduce the risk of infection among HCP as well as the risk of nosocomial transmission to patients.

[Children in the COVID-19 pandemic and the public health service (ÖGD) : Data and reflections from Frankfurt am Main, Germany]. / Kinder in der COVID-19 Pandemie und der Öffentliche Gesundheitsdienst (ÖGD) : Daten und Überlegungen aus Frankfurt am Main.

BACKGROUND: The measures taken to combat the COVID-19 pandemic have severely restricted the opportunities for the development of children. This paper will discuss the reporting data of children and the public health department's activities against the background of the restrictions of school and leisure time offers as well as sports and club activities. MATERIALS AND METHODS: Reporting data from Frankfurt am Main, Hesse, were obtained using a SURVStat query for the calendar weeks 10/2020-28/2021 and from SURVNet (until 30 June 2021). Contact persons (CP) of SARS-CoV­2 positive persons from schools and daycare centers were screened for SARS-CoV­2 by PCR test. These results and those of rapid antigen testing, which has been mandatory for schoolchildren since April 2021, are presented. RESULTS: Until Easter break, the age-related seven-day incidence values per 100,000 for children 14 years of age and younger were lower than the overall incidence; it was only higher after rapid antigen-testing was mandatory for schoolchildren. Most children with SARS-CoV­2 had no or mild symptoms; hospitalization was rarely required and no deaths occurred. Contact tracing in schools and daycare centers found no positive contacts in most cases and rarely more than two. Larger outbreaks did not occur. CONCLUSION: SARS-CoV­2 infections in children appear to be less frequent and much less severe than in adults. Hygiene rules and contact management have proven themselves effective during times with high incidences in the local population without mandatory rapid antigen testing - and even with a high proportion of variants of concern (alpha and delta variants) in Germany. Against this background, further restriction of school and daycare operations appears neither necessary nor appropriate.

Kinder in der COVID-19 Pandemie und der Öffentliche Gesundheitsdienst (ÖGD) : Daten und Überlegungen aus Frankfurt am Main Children in the COVID-19 pandemic and the public health service (ÖGD)Data and reflections from Frankfurt am Main, Germany

Hintergrund Die Maßnahmen zur Bekämpfung der COVID-19-Pandemie haben die Entwicklungsmöglichkeiten von Kindern stark eingeschränkt. In dem Beitrag werden die Meldedaten von Kindern und die Aktivitäten des Gesundheitsamtes vor dem Hintergrund der Einschränkungen der Schul- und Freizeitangebote sowie der Sport- und Vereinssportangebote diskutiert. Material und Methode Meldedaten aus Frankfurt am Main wurden mittels einer SURVStat-Abfrage (KW 10/2020–KW 28/2021) und aus SURVNet (bis 30.06.2021) ermittelt. Kontaktpersonen (KP) von SARS-CoV-2- positiv gemeldeten Personen aus Schulen und Kitas wurden mittels PCR-Test auf SARS-CoV‑2 untersucht. Die Ergebnisse sowie die der seit April 2021 für Schüler verpflichtenden Antigenschnelltests werden vorgestellt. Ergebnisse Die altersbezogenen 7‑Tage-Inzidenzen der Kinder bis 14 Jahre lagen vor der Einführung der Testpflicht für Schüler stets unter der Gesamtinzidenz, danach darüber. Die meisten Kinder mit SARS-CoV‑2 hatten keine oder nur milde Symptome;eine Hospitalisierung war selten erforderlich, Todesfälle traten nicht auf. Bei den Untersuchungen der KP in Schulen und Kitas wurden meist keine und nur selten mehr als 2 positive KP gefunden. Größere Ausbrüche traten nicht auf. Schlussfolgerung SARS-CoV-2-Infektionen bei Kindern sind offenbar seltener und deutlich weniger schwer als bei Erwachsenen. Größere Ausbrüche konnten im Setting Schule und Kita zuverlässig verhindert werden. Die AHA + L-Regeln und das Kontaktmanagement haben sich bewährt – auch bei hohen Inzidenzen in der lokalen Bevölkerung ohne Schnelltestpflicht und selbst bei einem hohen Anteil von besorgniserregenden Virusvarianten (Alpha und Delta) in Deutschland. Eine weitere Einschränkung des Schul- und Kitabetriebs scheint deshalb weder erforderlich noch angemessen zu sein.

Limited Neutralization of Authentic Severe Acute Respiratory Syndrome Coronavirus 2 Variants Carrying E484K In Vitro.

Whether monoclonal antibodies are able to neutralize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern has been investigated using pseudoviruses. In this study we show that bamlanivimab, casirivimab, and imdevimab efficiently neutralize authentic SARS-CoV-2, including variant B.1.1.7 (alpha), but variants B.1.351 (beta) and P.2 (zeta) were resistant against bamlanivimab and partially resistant to casirivimab. Whether antibodies are able to neutralize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variantshas been investigated using pseudoviruses. We show that authentic SARS-CoV-2 carrying E484K were resistant against bamlanivimab and less susceptible to casirivimab, convalescent and vaccine-elicited sera.

Antibody-Mediated Neutralization of Authentic SARS-CoV-2 B.1.617 Variants Harboring L452R and T478K/E484Q.

The capacity of convalescent and vaccine-elicited sera and monoclonal antibodies (mAb) to neutralize SARS-CoV-2 variants is currently of high relevance to assess the protection against infections. We performed a cell culture-based neutralization assay focusing on authentic SARS-CoV-2 variants B.1.617.1 (Kappa), B.1.617.2 (Delta), B.1.427/B.1.429 (Epsilon), all harboring the spike substitution L452R. We found that authentic SARS-CoV-2 variants harboring L452R had reduced susceptibility to convalescent and vaccine-elicited sera and mAbs. Compared to B.1, Kappa and Delta showed a reduced neutralization by convalescent sera by a factor of 8.00 and 5.33, respectively, which constitutes a 2-fold greater reduction when compared to Epsilon. BNT2b2 and mRNA1273 vaccine-elicited sera were less effective against Kappa, Delta, and Epsilon compared to B.1. No difference was observed between Kappa and Delta towards vaccine-elicited sera, whereas convalescent sera were 1.51-fold less effective against Delta, respectively. Both B.1.617 variants Kappa (+E484Q) and Delta (+T478K) were less susceptible to either casirivimab or imdevimab. In conclusion, in contrast to the parallel circulating Kappa variant, the neutralization efficiency of convalescent and vaccine-elicited sera against Delta was moderately reduced. Delta was resistant to imdevimab, which, however, might be circumvented by combination therapy with casirivimab together.

[Children and COVID-19-Data from mandatory reporting and results of contact person testing in daycare centers and schools in Frankfurt am Main, Germany, August-December 2020]. / Kinder und COVID-19: Kontaktpersonen-Surveillance in Frankfurter Kitas und Schulen (August bis Dezember 2020).

BACKGROUND: In face of the coronavirus disease 2019 (COVID-19) pandemic, the question that children are also drivers of this pandemic and that groups, classes, or the entire facility should be closed when severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs in schools or daycare centers is always implied. These questions were investigated using the mandatory reporting data in Frankfurt am Main and the extensive testing of contact persons (CP) in schools and daycare centers after the occurrence of an index case. METHOD: The reporting data were taken from SurvStat. The index cases from daycare centers and schools were isolated and the CPs were offered PCR testing for SARS-CoV­2 on a voluntary basis, regardless of whether symptoms suggestive of SARS-CoV­2 had occurred or not. Deep nasal/pharyngeal swabs were collected by paramedics on behalf of the public health department of the city of Frankfurt am Main, Germany, and tested according to established standards at two accredited institutes. RESULTS: From March to 31 December 2020, 22,715 COVID-19 cases were reported in Frankfurt, including 1588 (7.6%) SARS-CoV­2 detections in children 14 years and younger. Thus, approximately half as many SARS-CoV­2 detections were reported in children up to 14 years of age than would have corresponded to their proportion in the population. In autumn 2020, the increase in incidence in children over the weeks followed the increase in incidence in the general population, the age-related incidence of children remained below the incidence in the general population.From week 35 to week 52, index cases were reported from 143 daycare centers and 75 schools. As a result, 7915 CPs were tested. In daycare centers, SARS-CoV­2 was detected in 4.5% of adult CPs and 2.5% of child CPs and in schools SARS-CoV­2 was detected in 0.9% of adult CPs and 2.5% of student CPs tested. On average, less than 1 CP tested positive per index case. The rate of positive findings increased with increasing incidence in the overall population. No major outbreak occurred. DISCUSSION: Regarding the level and timing of age-related incidences among children in Frankfurt am Main, there was no evidence that children were the drivers of the pandemic. Only a small percentage of the examined CPs in schools and daycare centers tested positive for SARS-CoV­2. In the absence of evidence of intense transmission in the facilities, CP attendance can/should continue under hygiene conditions and there is no need to close entire groups, classes, or even facilities.

Surveillance of SARS-CoV-2 in Frankfurt am Main from October to December 2020 Reveals High Viral Diversity Including Spike Mutation N501Y in B.1.1.70 and B.1.1.7.

BACKGROUND: International travel is a major driver of the introduction and spread of SARS-CoV-2. AIM: To investigate SARS-CoV-2 genetic diversity in the region of a major transport hub in Germany, we characterized the viral sequence diversity of the SARS-CoV-2 variants circulating in Frankfurt am Main, the city with the largest airport in Germany, from the end of October to the end of December 2020. METHODS: In total, we recovered 136 SARS-CoV-2 genomes from nasopharyngeal swab samples. We isolated 104 isolates that were grown in cell culture and RNA from the recovered viruses and subjected them to full-genome sequence analysis. In addition, 32 nasopharyngeal swab samples were directly sequenced. RESULTS AND CONCLUSION: We found 28 different lineages of SARS-CoV-2 circulating during the study period, including the variant of concern B.1.1.7 (Δ69/70, N501Y). Six of the lineages had not previously been observed in Germany. We detected the spike protein (S) deletion Δ69/Δ70 in 15% of all sequences, a four base pair (bp) deletion (in 2.9% of sequences) and a single bp deletion (in 0.7% of sequences) in ORF3a, leading to ORF3a truncations. In four sequences (2.9%), an amino acid deletion at position 210 in S was identified. In a single sample (0.7%), both a 9 bp deletion in ORF1ab and a 7 bp deletion in ORF7a were identified. One sequence in lineage B.1.1.70 had an N501Y substitution while lacking the Δ69/70 in S. The high diversity of sequences observed over two months in Frankfurt am Main highlights the persisting need for continuous SARS-CoV-2 surveillance using full-genome sequencing, particularly in cities with international airport connections.

Sars-Cov-2 in children - insights and conclusions from the mandatory reporting data in Frankfurt am Main, Germany, March-July 2020.

Introduction: From the beginning of the corona pandemic until August 19, 2020, more than 21,989,366 cases have been reported worldwide - 228,495 in Germany alone, including 12,648 children aged 0-14. In many countries, the proportion of infected children in the total population is comparatively low; in addition, children often have no or milder symptoms and are less likely to transmit the pathogen to adults than the other way round. Based on the registration data in Frankfurt am Main, Germany, the symptoms of children in comparison with adults and the likely routes of transmission are presented below. Materials and methods: The documentation of the mandatory reports includes personal data (name, date of birth, gender, place of residence), disease characteristics (date of report, date of onset of the disease, symptoms), possible contact persons (family, others) and i.a. possible activity or care in children's community facilities. All reports were viewed, especially with regard to likely transmission routes. Results: From March 1 to July 31, 2020, 1,977 infected people were reported, including 138 children between the ages of 0 and 14 years. Children had fewer and milder symptoms than adults. None of the children experienced severe respiratory symptoms or the need for ventilation. 62% of the children had no symptoms at all (19% adults), 5% of the children were hospitalized (24% adults), and none of the children died (3.8% adults). After excluding a cluster of 34 children from refugee accommodations and 14 children from a parish, 78% of the remaining 90 children had been infected by an adult within the family, and only 4% were likely to have a reverse transmission route. In 5.5% of cases, transmission in a community facility was likely. Discussion: The results of the registration data from Frankfurt am Main, Germany confirm the results published in other countries: Children are less likely to become infected, and if infected, their symptoms are less severe than in adults, and they are apparently not the main drivers of virus transmission. Therefore, scientific medical associations strongly recommend reopening schools.

[Mandatory notification of infectious diseases and agents in Germany: development and suggestions for improvement]. / Meldepflichten für Infektionskrankheiten und Infektionserreger in Deutschland: Entwicklung und Verbesserungsvorschläge.

With the entry into force of the Infection Protection Act (IfSG) in 2001, the reporting obligations for infectious diseases and infectious agents were placed on a new foundation. For the first time, a distinction was made between an obligation for the notification of infectious diseases by physicians and a notification obligation for infectious agents by laboratories. The aim was to reduce the notification burden on physicians and thus to improve the quality of the notifications. Since then, numerous new obligations for notifications have been added.The aim of this work is to describe and discuss the mandatory notification of infectious diseases in Germany on the basis of their development - compared to previous regulations in Germany (Federal Communicable Diseases Act) as well as international and Europe-wide recommendations (IHR; decisions of the EU Commission 1999, 2018) - and to submit suggestions for improvement.Regarding the considerable increase in reporting requirements and reports in recent years, and the fact that the IfSG provides other surveillance systems in addition to mandatory reporting, the mandatory reporting system should be focused on the necessary reporting requirements. In a first step, the proposed abolition of the mandatory reporting of noroviruses and rotaviruses could relieve both the notifiers and the health authorities, thus enabling more efficient reporting and more intensive and better investigation by the health authorities.